Research on the mechanism of Srolo Bzhtang intervention in MCT-induced pulmonary arterial hypertension in rats based on network pharmacology
Objective Research on the mechanism of Srolo Bzhtang intervention in MCT-induced pulmonary arterial hypertension in rats based on network pharmacology.Methods The Swiss Target Prediction database was used to predict potential targets of the component absorbed into the blood of SBT.The network of"component ab-sorbed into blood-target"was constructed.The enrichment of GO and KEGG were analyzed based on the David platform.A model of PAH rat was made with monocrotaline.The intervention effect of SBT was evaluated by hemody-namic and morphological methods.Western Blotting was used to detect the protein expressions of PPAR-γ,Smad3,α-SMA,Cyclind1,P27,CDK4 and BMPR2 in lung tissue.Results The results of GO and KEGG enrichment analysis showed that SBT mainly played a role through PPAR-γ signaling pathways.The results of experimental verification in vivo confirmed that SBT could effectively reduce pulmonary artery pressure and relieve pulmonary vascular re-modeling in PAH rats.Mechanism verification showed that SBT could up-regulate PPAR-γ and down-regulate Smad3.Meanwhile,it could inhibit the expression of proliferation related protein Cyclin d1 and upregulate the ex-pression of protein P27.Conclusion SBT could inhibit the proliferation of pulmonary artery smooth muscle cells and improve pulmonary vascular remodeling by activating the BMPR2/PPAR-γ/Smad3 signaling pathways.
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