Changes of hepatic ACC1 and CPT1A and their effects on glucose and lipid metabolism in male mice at medium and high altitude
Objective To investigate the changes of hepatic ACC1 and CPT1A and their effects on glucose-lipid metabolism in male mice at medium and high altitude.Methods Male mice were randomly divided into medi-um altitude group(M group)and high altitude group(H group),and then divided into 3d,7d and 30d groups accord-ing to the number days of reading,with 10 mice in each group.On the3rd,7th and 30th day,the serum of the mice was taken to determine insulin.Liver tissue were taken to determine CPT1A messenger RNA and protein level and ACC1 messenger RNA and protein level by PCR and WB method.Results 1)Within a certain time range,fasting blood glucose and fasting insulin levels in M group gradually increased with the extension of time,but fasting blood glucose and fasting insulin levels in H group gradually decreased and were lower than in M group at the same time period(P＜0.05).2)CPT1A messenger RNA levels gradually decreased in M group with time extension,while there was no significant change in H group with time extension and were lower than the levers in M group at the same pe-riod(P＜0.05),and there was no difference between M group and the H group at the 30 d;ACC1 messenger RNA gradually increased with time extension in M group,while gradually decreased with time extension in H group and was significantly lower in M group than in H group on the 3 d.CPT1A protein levels gradually decreased with time in M group and in H group,and H group was lower than M group in the same period(P＜0.05);ACC1 protein levels gradually increased M group and gradually decreased in H group with time extension(P＜0.05).3)CPT1A messen-ger RNA and protein levels were positively correlated with fasting insulin levels,insulin resistance levels,and insu-lin secretion index levels(P＜0.05);ACC1 messenger RNA levels were negatively correlated with fasting insulin levels,insulin resistance levels,and insulin secretion index levels(P＜0.05);ACC1 messenger RNA levels were neg-atively correlated with fasting insulin levels,insulin resistance levels,and insulin secretion index levels(P＜0.05);ACC1 protein level was positively correlated with fasting glucose level and negatively correlated with insulin secre-tion index level(P＜0.05).Conclusion 1)Medium altitude decreased hepatic ACC1 messenger RNA and protein levels,but increased CPT1A messenger RNA and protein levels in male mice;high altitude increased hepatic ACC1 messenger RNA and protein,while decreased CPT1A messenger RNA and protein levels in male mice.2)The glu-cose metabolism and hepatic fatty acid synthesis metabolism were increased in male exposed to acute hypoxia at high altitude.With the prolongation of hypoxia,glucose metabolism was gradually converted to fatty acid synthesis metabolism,which was manifested by decreased blood glucose,decrease hepatic fatty acid and enhancement of ca-tabolism.
AltitudeMaleMiceLiverAcetyl coenzyme A carboxylase 1Carnitine palmitoyltransferase 1AGlucoseLipidMetabolismEffects
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