Exploration of the effects of Trichosanthes kirilowii Maxim.on the hypoxia-induced proliferation and migration of pulmonary arterial smooth muscle cells based on network pharmacology and in vitro experiments
Objective To investigate the effects of Trichosanthes kirilowii Maxim.on the hypoxia-induced pro-liferation and migration of rat pulmonary artery smooth muscle cells(PASMCs)by adopting network pharmacology and in vitro experiments.Methods Various databases,including TCMSP,Pubchen,Swiss Target Prediction,Gene-Cards,OMIM,DisGeNET,STRING,PDB,were utilized to predict potential target genes of Trichosanthes kirilowii Maxim.in hypoxic pulmonary hypertension.Core target genes were identified for enrichment analysis.Molecular docking technology was utilized to confirm the prediction results of network pharmacology.In vitro experiments were carried out to evaluate the effects of Trichosanthes kirilowii Maxim.extract on the hypoxia-induced prolifera-tion and migration of rat PASMCs.Results A total of 210 potential target genes of Trichosanthes kirilowii Maxim.against hypoxic pulmonary hypertension were identified after integrating multiple databases.Further screening re-vealed 43 core target genes,including IL6,ESR1,AKT1 and BCL2.The results of GO and KEGG enrichment analy-ses indicated that Trichosanthes kirilowii Maxim.exerted its anti-hypoxic pulmonary hypertension effects by modu-lating PI3K/AKT,FoxO,Ras and other signaling pathways.Molecular docking demonstrated that various components in Trichosanthes kirilowii Maxim.exhibit strong binding abilities with different target genes.In vitro experiments re-vealed that the extract of Trichosanthes kirilowii Maxim.effectively inhibits the hypoxia-induced proliferation and migration of rat PASMCs.Conclusion The extract of Trichosanthes kirilowii Maxim.may inhibit the hypoxia-in-duced proliferation and migration of rat pulmonary artery smooth muscle cells through multiple components,multi-ple targets and multiple pathways,thereby exerting a protective effect on hypoxic pulmonary arterial hypertension.
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