Mechanism of aloperine inhibiting the proliferation of HGC-27 and AGS gastric cancer cells
Objective To explore the mechanism of aloperine(ALO)inhibiting the proliferation of HGC-27 and AGS gastric cancer cells.Methods HGC-27 cells and AGS cells were exposed to varying concentrations of ALO.Proliferative activities were assessed by means of CCK-8 experiments,cell proliferation experiments and plate cloning experiments.Migration and repair ability were evaluated through scratch experiments.Migration and invasion abilities were measured through transwell experiments.Flow cytometry was adopted to analyze the varia-tions of cell cycle.The expression of mRNA levels of GSPT1,CDK4,Cyclin DI and Caspase-3,Caspase-9,Bax,Bcl-2 were determined by using qTR-PCR.Western Blot was performed to assess the protein expression levels of GSPT1,Caspase-3,Caspase-9,Bax and Bcl-2.Results The results of CCK-8 experiments,cell proliferation ex-periments and plate cloning experiments showed that the proliferation of HGC-27 and AGS gastric cancer cells were inhibited by ALO in a concentration dependent manner.That is to say,compared with the control group,the proliferation ability of HGC-27 cells and AGS cells treated with 0.1 mM and 0.25 mM ALO decreased.The re-sults of scratch experiments showed that the migration and repair ability of HGC-27 cells and AGS cells were inhibited by ALO.The results of transwell experiments showed that the migration and invasion ability of HGC-27 cells and AGS cells treated with ALO were inhibited.The results of flow cytometry showed that there were variations in the cell cycle of HGC-27 and AGS after ALO treatment.The results of qRT-PCR and Western Blot showed that compared with the control group,the expression levels of GSPT1、Bcl-2,CDK4 and Cyclin DI in HGC-27 cells and AGS cells treated with drugs decreased,while that of Caspase-3,Caspase-9,Bax increased.Conclusion ALO may inhibit the proliferation of HGC-27 and AGS gastric cancer cells by regulating the ex-pression levels of GSPT1,Caspase-3,Caspase-9,Bax and Bcl-2.
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