Effects of high-altitude hypoxia on learning and memory in male rats and the expression of NR2A,P-NR2A and P-NR2B in their frontal lobe
Objective To investigate the effects of high-altitude hypoxia on learning and memory in male rats,as well as the expression of NR2A,P-NR2A and P-NR2B in their frontal lobe.Methods A total of 48 SPF SD rats were randomly divided into normoxia group(1D,5D and 12D groups)and hypoxia group(1D,5D and 12D groups),totalling 6 groups(8 rats/group).The learning and memory ability of rats was evaluated by water maze method.The morphology of nerve cells in frontal lobe tissue was observed by HE staining.The expression of NR2A,P-NR2A and P-NR2B was detected by Western Blot.Results The results of water maze experiment showed that with the in-crease of training times,the distance of rats finding the platform was gradually shortened;with the prolongation of forgetting time,the number of rats crossing the target quadrant of the platform decreased.The results of HE staining showed that the morphological structure of frontal lobe neurons in each group was complete.There was no signifi-cant difference in the morphological structure and number of neurons between the normoxia group and the hypoxia group.Compared with the normoxia group at the same time point,the expression of NR2A in the hypoxia group was significantly increased on the first and fifth days,while decreased on the twelfth day.The difference was statistically significant(P＜0.005).The expression of P-NR2A and P-NR2B in the hypoxia group was significantly increased(P＜0.005).Conclusion Hypoxia has a certain damage to the learning and memory of male rats,possibly by down-regulating the expression of NR2A and up-regulating the expression of P-NR2A and P-NR2B proteins.
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