Objective To investigate the expressions of mucin 5AC(MUC5AC)and mucin 5B(MUC5B)in patients with chronic sinusitis and their relationship with bacterial biofilm formation.Methods Retrospective analysis was con-ducted on 50 patients with chronic sinusitis who underwent endoscopic sinus surgery from December 2020 to April 2023 and 50 healthy specimens preserved by pathology.Immunofluorescence and immunoblotting were used to detect the ex-pression of MUC5AC,MUC5B,and protein in nasal mucosa tissue.Laser scanning confocal microscopy was used to ob-serve the positive expressions of bacterial biofilm and Lund-Kennedy score was performed.The morphology of bacterial biofilm was observed under scanning electron microscopy.Pearson method was used to analyze the correlation between MUC5AC,MUC5B,and bacterial biofilm positive score.Results The expressions of MUC5AC and MUC5B positive cells in nasal mucosa tissues of observation group were higher than that of control group,and the differences were statisti-cally significant(t=54.27,61.18,P＜0.05).The expressions of MUC5AC and MUC5B proteins in the nasal mucosa tissue of the observation group were higher than that of the control group,and the differences were statistically significant(t=43.51,70.90,P＜0.05).The bacterial biofilm positive score in observation group was higher than that in control group,and the difference was statistically significant(t=8.96,P＜0.05).Scanning electron microscopy showed that the mucosal tissue surface of the observation group patients was accompanied by adhesive aggregation,lodging,and some tissues were lost,while the control group muco-sa had no significant pathological reactions.Both MUC5AC and MUC5B were positively correlated with bacterial biofilm positive scores(r=0.37,0.30,P＜0.05).Conclusion The MUC5AC,MUC5B in chronic sinusitis is significantly positively correlated with bacterial biofilm formation.Strengthening the detection of MUC5AC and MUC5B in clinical diagnosis and treatment is of great significance for disease detection and the implementation of subsequent treatment plans.
维普
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