Objective We utilized microarray and quantitative PCR(qRT-PCR)techniques to evaluate the diag-nostic potential of miRNAs in heart failure.Methods We used microarray to explore the miRNA expression profile in the serum of heart failure patients and evaluated the expression levels of candidate miRNAs using the qRT-PCR method.The diagnostic value of candidate miRNAs was analyzed using receiver operating characteristic(ROC)curve.Results Compared with the healthy control,miR-125a-5p and miR-99b-5p were downregulated in the serum exo-somes of patients with heart failure,the areas under the ROC curve(AUC)was 0.73 and 0.72,respectively.Further-more,the combined detection of miR-125a-5p and miR-99b-5p has higher diagnostic value than individual miR-NAs,with an AUC of 0.80.Conclusion The expression levels of miR-125a-5p and miR-99b-5p are significantly downregulated in the serum exosomes of heart failure patients,potentially serving as novel circulating biomarkers for the diagnosis of heart failure.The diagnostic performance of miR-125a-5p combined with miR-99b-5p is higher than individual index.
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