摘要
目的 探究银杏叶黄酮通过下调急性脑梗死小鼠血清肿瘤坏死因子-α(TNF-α)、半胱天冬酶(Caspase-3)及低氧诱导因子-1α(HIF-1α)表达对抑制神经细胞凋亡的影响.方法 选择健康清洁级6 周龄雌性昆明小鼠45只,随机分为观察组、模型组和假手术组,每组15只.观察组和模型组小鼠建模成功后,模型组给予腹腔注射0.9%的氯化钠注射液,连续注射1 周;观察组给予腹腔注射银杏叶黄酮注射液20 mg/kg,连续注射1 周.比较三组小鼠脑梗死面积、神经细胞凋亡率,以及TNF-α、Caspase-3、HIF-1α水平和蛋白相对表达量.结果 模型组大脑皮质神经元细胞结构功能不完整,突触小泡、细胞器数目减少,核膜断裂,染色质凝集成块.观察组小鼠大脑皮质神经元细胞损伤程度轻于模型组,仅少数细胞呈现固缩和空泡化现象.与假手术组比较,观察组和模型组小鼠TNF-α、Caspase-3及HIF-1α水平明显升高(t分别=3.89、4.68、5.23、4.67、4.21、4.71,P均＜0.05);且与模型组比较,观察组小鼠TNF-α、Caspase-3及HIF-1α水平明显降低(t分别=4.04、4.66、3.66,P均＜0.05).与假手术组比较,模型组和观察组小鼠TNF-α、Caspase-3及HIF-1α蛋白表达量明显升高(t分别=3.98、4.56、4.18、4.39、3.67、4.29,P均＜0.05);且与模型组比较,观察组小鼠TNF-α、Caspase-3及HIF-1α蛋白表达量明显降低(t分别=5.03、4.32、6.30,P均＜0.05).与假手术组比较,观察组和模型组小鼠梗死面积、神经细胞凋亡率明显升高(t分别=4.56、4.98,P均＜0.05);且与模型组比较,观察组小鼠梗死面积、神经细胞凋亡率明显降低(t分别=4.38、6.39,P均＜0.05).结论 银杏叶黄酮通过下调急性脑梗死小鼠TNF-α、Caspase-3及HIF-1α表达来抑制神经细胞凋亡.
Abstract
Objective To explore the effect of Ginkgo biloba flavone on inhibiting neuronal apoptosis by down regulat-ing the expression of serum TNF-α,caspase-3 and HIF-1 α in mice with acute cerebral infarction.Methods Totally 45 healthy and clean 6-week-old female Kunming mice were selected,and randomly divided into observation group,mod-el group and sham operation group,with 15 mice in each group.After successfully modeled,the model group was given intraperitoneal injection of 0.9%normal saline for 1 week.The observation group was given intraperitoneal injection of Ginkgo biloba flavone injection 20 mg/kg for 1 week.The cerebral infarction area,apoptosis rate of nerve cells,the con-tents of tumor necrosis factor-α(TNF-α),caspase-3 and hypoxia inducible factor-1α(HIF-1α)and the relative protein expression of TNF-α,caspase-3 and HIF-1α were compared among the three groups.Results The structure and func-tion of cortical neurons in the model group are incomplete,with a decrease in synaptic vesicles and organelles,nuclear membrane rupture,and chromatin aggregation into blocks.The degree of damage to cortical neurons in the observation group was milder than that in the model group,with only a few cells showing consolidation and vacuolization.Compared with the sham surgery group,the levels of TNF-α,Caspase-3,and HIF-1α in the observation group and model group were significantly increased(t=3.89,4.68,5.23,4.67,4.21,4.71,P＜0.05).Compared with the model group,the levels of TNF-α,Caspase-3,and HIF-1α in the observation group were significantly reduced(t=4.04,4.66,3.66,P＜0.05).Compared with the sham surgery group,the expression levels of TNF-α,Caspase-3,and HIF-1α proteins in the model group and observation group were signifi-cantly increased(t=3.98,4.56,4.18,4.39,3.67,4.29,P＜0.05).Compared with the model group,the expression levels of TNF-α,Caspase-3,and HIF-1α proteins in the observation group were significantly reduced(t=5.03,4.32,6.30,P＜0.05).Compared with the sham surgery group,the apoptosis rate of nerve cells in the observation group and model group was significantly increased(t=4.56,4.98,P＜0.05).Compared with the model group,the infarct size and neuronal apopto-sis rate of the observation group were significantly reduced(t=4.38,6.39,P＜0.05).Conclusion Ginkgo biloba flavonoids inhibit neuronal apoptosis by down regulating the expression of TNF-α,Caspase-3,and HIF-1α in mice with acute cere-bral infarction.
维普
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