Objective:To detect the role of miR-155-5P expression in evaluating prognosis of patients with acute myeloid leukemia(AML).Methods:The qRT PCR method was used to detect the expression ofmiRNA-155-5P in peripheral blood mononuclear cells of AML patients after 2 weeks of induction therapy.Based on the cut-off value,patients were divided into two groups,such as greater than the cut-off value and less than the cut-off value.According to the 2017 ELN efficacy criteria,the complete remission group(CR)and the non remission group(NR)were confirmed.The significance of statistical analysis of miR-155-5P expression levels in peripheral blood mononuclear cells after 2 weeks of treatment in evaluating the survival rate,recurrence rate,minimal residual disease,and disease-free survival of AML patients.Results:At 2 weeks of chemotherapy induced treatment,the expression levels of miRNA-155-5P in peripheral blood mononuclear cells of AML patients were higher than those of the normal control group(t=2.812,P=0.0001).The expression of miRNA-155-5P in peripheral blood mononuclear cells of the CR group was significantly lower than that of the NR group(t=3.407,P=0.0012),while the expression of miRNA-155-5P in peripheral blood mononuclear cells of the recurrent group was higher than that of the non recurrent group(t=4.293,P＜0.001).There was no significant correlation between miRNA-155-5P levels in peripheral blood mononuclear cells and disease-free survival(DFS)(r=0.1600,P=0.265).However,there was a significant difference in disease-free survival between the two groups with miRNA-155-5P greater than and less than the cut-off value(t=4.54,P＜0.001).The incidence of MRD and NR in the group above the critical value were lower than those in the group below the cut-off value(x2=7.810,P=0.011;x2=10.083,P=0.001).However,there was no significant difference in the CR rate and recurrence rate between the two groups with miRNA-155-5P greater than and less than the cut-off value(x2=3.176,P=0.075;x2=1.539,P=0.215).AML patients with miRNA-155-5P lower than the cut-off value group had higher 3-year and 5-year survival rates(x2=12.84,P=0.0003;x2=10.81,P=0.0010).Conclusion:The expression level of miR-155-5P in peripheral blood mononuclear cells after 2 weeks of chemotherapy induction therapy is an important biological indicator for the clinical prognosis of AML patients.
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