DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN ORAL SQUAMOUS CELL CARCINOMA
Objective To investigate the infection of human papillomavirus (HPV) in oral squamous cell carcinoma (SCC).Methods Fifty three patients with oral SCC treated in our department between July 2014 and July 2016-were served as the experimental group.The cancer tissue,paracancerous tissue and normal oral mucosa were collected from the patients.Normal oral mucosa was taken from 50 patients with non-oral SCC treated in our department and 50 healthy volunteers-they were served as control group.The HPV infection of samples in the two groups was detected applying HPV DNA genotyping with PCR-Reverse Dot Blot hybridization technique.Results In the experimental group,HPV positive was found in eight (15 ％) cases,of which,one of mixed infection with high risk and low risk,and seven of HPV high risk single infection.In the control group,two cases (1.0 ％) were found of PPV positive,both of mixed infection with high and low risk,the difference in HPV infection rate be tween the two groups was significant (x2 =37.5,P＜0.05).In patients with oral SCC of positive HPV,four of stage Ⅰ-Ⅱ and four of stage Ⅲ-Ⅳwere found;of HPV negative,25 of stage Ⅰ-Ⅱ,20 of stage Ⅲ-Ⅳ,the difference was not significant between the two groups (P＞0.05).In patients with oral SCC of HPV positive,cervical lymph node metastasis was found in three patients,and five without metastasis;those with oral SCC of negative HPV,20 were found with cervical lymph node metastasis,and 25 without,the difference between the two groups was not significant (P＞0.05).Conclusion The infection rate of HPV in patients with oral squamous cell carcinoma is higher than that in normal population and other oral-disease population.HPV infection is not correlated with clinical stage and lymphnode metastasis of oral squamous cell carcinoma.
NETL
NSTL
万方数据
维普
