EFFECT OF EPIGALLOCATECHIN GALLATE ON THE EXPRESSION OF GABAA RECEPTOR IN HIPPOCAMPUS OF CHRONIC EMOTIONAL STRESS-INDUCED MICE
Objective To study the effects of epigallocatechin gallate (EGCG) on the expression of GABAA receptor in hippocampus of chronic emotional stress-induced mice.Methods Sixty male SPF Kunming mice were evenly randomized to normal control group,model group,Diazepam group,high,moderate and low-EGCG-dose groups.The mice in normal control group were not specially treated,drinking and feeding freely as usual;those in the model group were given isometric normal saline;those in Diazepam group were given Diazepam 5 mg/(kg · d),and those in the high,moderate-and low-EGCG dose groups were given 60,30 and 15 mg/(kg · d) of EGCG,respectively.An intragastric administration was conducted in rats of each group.On the end of the forth week,the anxious behavior of the mice was tested adopting Elevated Plus-maze.Employing immunohistochemistry,the expression of GABAA receptor in CA1 and CA3 region of hippocampus of mice was detected.Results Compared with the model group,the time spent in the open arms (OT％),the percentage of open arms entries (OE％) in normal control group,diazepam group and moderate EGCG dose group were significantly increased (F=2.02,5.26;q=3.23 4.60;P＜0.05).The number of GABAA receptor positive cells in CA1 and CA3 of hippocampal area increased remarkably (F=2.86,5.13;q =3.22-4.63;P ＜0.05);the OE％,OT％ and GABAA receptor positive cells in hippocampi of low-and high-EGCG dose groups showed an increased trend,but the difference was not significant as compared with the model group (P＞0.05).Conclusion Epigallocatechin gallate has a significant anti anxiety effect,the most obvious effect is in its middle dose,presumably,it plays a role in anti anxiety may be by increasing the expression of GABAA.
epigallocatechin gallatechronic emotional stresshippocampusγ-aminobutydc acid A receptor
NETL
NSTL
万方数据
维普
