EFFECT OF MONOSODIUM URATES CRYSTALS ON THE PROLIFERATION AND DIFFERENTIATION OF 3T3-E1 CELLS
Objective To observe the effect of monosodium urates (MSU) on the proliferation and differentiation of 3T3-E1 cells.Methods 3T3-E1 cells were induced to differentiate into osteoblast with different concentrations of MSU-0,0.02,0.06,0.10,0.20,0.60 g/L.After 48 h of stimulation culture,growth of the cells in each group was observed applying MTT method.3T3 E1 cells were taken and osteogenic induce supplement added.MSU was not added to the control group.MSU-0.02 and 0.10 g/L-was added to the experimental group.After 14h of induction,alkaline phosphatase staning was done and RNA samples were extracted.Applying RNA real time fluorecent quantitative PCR,the expressions of Runx 2,Osterir,Osteocalcin,and Collal 1 were detected.Results MSU could inhibit 3T3 E1 proliferation,it became strong with the increase of its concentration (F =560.74,q=8.52-63.52,P＜0.05).The alkaline phosphatase staining revealed the color became lighter with the increase MSU concentration.RT-PCR indicated that the expressions of Runx2,Osterix,Osteocalcin and Collal 1 declined with the increase of MSU concentration (F=31.06-112.47,q=3.82-20.57,P＜0.05).Conclusion Monosodium urates can inhibit osteoblast precursor cell (3T3-E1) proliferation and its differentiation into osteoblast and secretion.
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