EFFECTS OF SLEEP DEPRIVATION AND RECOVERY ON THE EXPRESSION OF GAT-1 IN HIPPOCAMPUS OF RATS
Objective To investigate the effects of sleep deprivation and recovery on the expression of γ-aminobutyric acid transporter 1 (GAT-1) in hippocampus of rats.Methods Twenty four healthy male Sprague Dawley rats were equally randomized to three groups as normal control group (group C),sleep deprivation group (group D) and sleep deprivation + recovery group (group D+R).Morris water maze traing was conducted in rats of each group before the experiment.The rats in group C received Morris water maze test on the fifth day of the experiment.The rats in group D were deprivated from sleeping for 96 h and then received Water maze test,those in group D+R were deprivated from sleeping for 96 h,followed by recovery of sleep for 12 h,and then Water maze test carried out.The rats were executed upon completion of the test.Using immunohistochemistry method,the expression of GAT 1 in the hippocampus CA1 region was detected.Results Compared with before the experiment,the escape latency in group D and group D+R was prolonged (t =-5.714,3.069;P＜0.05).After the experiment,compared with group C,the escape latency in groups D and D+ R prolonged (F =21.535;q=9.23,3.76;P ＜0.05),the frequency of crossing the original platform decreased (F=19.096;q=8.72,3.88;P＜0.05),and the number of GAT-1 positive cells in the hippocampus CA1 region in group D decreased (F=21.664,q=4.77,P＜0.05).Compared with group D,the escape latency in group D+R was shor-tened (q=5.48,P＜0.05),the frequency of crossing the original platform and the number of GAT-1 positive cells in the hip pocampal CA1 region increased in group D+R after sleep recovery (q 4.85,4.54;P＜0.05).Before the experiment,the difference in the escape latency among the three groups was not significant (P＞0.05).Conclusion The effect of sleep deprivation and recovery on the spatial learning and memory ability of rats is related to the expression of GAT-1 in hippocampus.
sleep deprivationhippocampusgamma-aminobutyric acidamino acid transport systerm
NETL
NSTL
万方数据
维普
