APPLICATION OF GENECHIP MICROARRAYS IN THE STUDY ON THE MECHANISM OF ISATIN INHIBITING NEUROBLASTOMA
Objective To investigate the underlying mechanism behind Isatin's ability to inhibit neuroblastoma using GeneChip microarrays,and to identify the possible targets associated with neuroblastoma cell invasion and metastasis.Methods Human neuroblastoma cells (SH-SY5Y) were exposed to Isatin at different concentrations in vitro.The effect of Isatin on the viability of SH-SY5Y cells was determined by CCK8 assay,and the effect of Isatin on the gene expression profile in SH SY5Y cells using Affymetrix GeneChip microarrays.The GeneGo was used for Gene Ontology (GO) analysis.Two databases,BIOCARTA and KEGG,were used to filter out the key genes and pathways associated with the neuroblastoma cell invasion and metastasis.Results The half-maximal inhibitory concentration of Isatin for SH-SY5Y cells was 279.7 μmol/L.There were 284 up-regulated genes and 145 down-regulated genes in Isatin-treated SH SY5Y cells.GO analysis showed that Isatin inhibited SH-SY5Y cell proliferation,cell cycle,cellular translation,biosynthesis,and metabolic function.According to the KEGG and BIOCARTA analyses,we found that the mammalian target of rapamycin (mTOR) signaling pathway might be related to neuroblastoma cell invasion and metastasis,DDIT4,RHEB,EIF4EBP1,and RPS6KB1 were key genes involved in the mTOR signaling pathway.Conclusion Isatin inhibits SH SY5Y neuroblastoma cell invasion and metastasis through the mTOR signaling pathway.
NETL
NSTL
万方数据
维普
