首页|LPS和NNK对大鼠骨髓间充质干细胞增殖及NF-κB、P-P38、CUGBP1蛋白表达的影响

LPS和NNK对大鼠骨髓间充质干细胞增殖及NF-κB、P-P38、CUGBP1蛋白表达的影响

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的 观察炎性刺激因子脂多糖(LPS)、4-(甲基亚硝氨基)-1-(3-吡啶基)-1-丁酮(NNK)及LPS联合NNK对体外培养大鼠骨髓间充质干细胞(BMSCs)增殖及核因子κB(NF-κB)、P-P38、CUGBP1蛋白表达的影响.方法 应用全骨髓贴壁法体外培养纯化BMSCs.采用MTT法检测不同浓度的炎性因子对BMSCs增殖的影响,选择最佳刺激浓度.应用免疫细胞化学法检测炎性刺激对BMSCs中NF-κB、CUGBP1蛋白表达的影响,采用Western blot法检测炎性刺激对BMSCs中NF-κB、P-P38、CUGBP1蛋白表达的影响.结果 不同浓度的炎性因子均能促进大鼠BMSCs增殖,其中以12.5 mg/L LPS、10 mg/L NNK、12.5 mg/L LPS联合10 mg/L NNK对大鼠BMSCs增殖的促进作用最为明显.免疫细胞化学法检测显示,炎性刺激后BMSCs中NF-κB、CUGBP1蛋白的表达量显著增加,差异有显著性(F=165.1～481.8,P＜0.01).Western blot法检测显示,炎性刺激后NF-κB、P-P38、CUGBP1蛋白的表达量增加,差异有统计学意义(F=38.1～1 531.0,P＜0.01).结论 炎性因子可能通过激活P38MAPK和NF-κB信号途径促进大鼠BMSCs的增殖.长时间的炎性因子刺激,促进了NF-κB、P-P38、CUGBP1蛋白在细胞核中的表达.
EFFECTS OF LPS AND NNK ON PROLIFERATION OF BONE MARROW MESENCHYMAL STEM CELLS AND THE EXPRESSION LEVELS OF NF-κB, P-P38, AND CUGBPI IN RATS
Objective To observe the effects of lipopolysaccharide (LPS),4-(methylnitrosamino)-1-(3-pyridyl)-1 butanone (NNK),and a combination of LPS and NNK on the proliferation of bone marrow mesenchymal stem cells (BMSCs) and expression levels of NF-κB,P-P38 and CUGBP1 in rats.Methods Whole bone marrow adherent culture was used to culture and purify BMSCs.The effect of different concentrations of inflammatory factors on the proliferation of BMSCs was evaluated by MTT assay,based on which the stimulating concentration was optimized.The expression levels of NF κB,P-P38,and CUGBP1 in BMSCs were measured by immunocytochemistry and Western blot.Results Different concentrations of inflammatory factors could all promote the proliferation of rat BMSCs,and 12.5 mg/L LPS,10 mg/L NNK,and 12.5 mg/L PLS in combination with 10 mg/L NNK had the most significant promotional effects on the proliferation of rat BMSCs.Immunocytochemistry revealed that after inflammatory stimulation,NF-κB and CUGBP1 protein levels in BMSCs were significantly increased (F =165.1-481.8,P＜0.01).Western blot showed that the expression levels of NF-κB,P P38,and CUGBP1 in BMSCs were increased after inflammatory stimulation (F=38.1-1 531.0,P＜0.01).Conclusion LPS,NNK,and LPS in combination with NNK may promote the proliferation of BMSCs by activating NF-κB and P38MAPK pathways.Long-term inflammatory factor stimulation promotes the expression of NF-κB,P-P38,and CUGBP1 in cell nucleus.

lipopolysaccharidesbutanonesmesenchymal stem cellsbone marrowcell proliferationNF-kappa BP38 mitogen-activated protein kinasesCUGBP1

姜莎莎、王怡、于忠杰、荆昭、刘晓萍、狄元璞

展开 >

青岛大学医学院组织学与胚胎学教研室,山东青岛266021

美国匹兹堡大学环境与职业健康学院

脂多糖类 丁酮类 间质干细胞 骨髓 细胞增殖 NF-κB P38丝裂原活化蛋白激酶类 CUGBP1

山东省自然科学基金

ZR2012CM008

2017

10.13362/j.qlyx.201702005

齐鲁医学杂志
青岛大学医学院

齐鲁医学杂志

影响因子：0.609
ISSN：1008-0341
年,卷(期)：2017.32(2)
  • 8