EFFECTS OF PHYTIC ACID ON EXPRESSION OF ZO-1 , OCCLUDIN, AND CLAUDIN-1 IN WISTAR RATS WITH COLORECTAL CANCER INDUCED BY DIMETHYLHYDRAZINE
Objective To observe the effects of different doses of phytic acid (PA) on the expression of zo-1,occludin,and claudin-1 in rats with colorectal cancer (CRC),and to investigate the effects of PA on the intestinal mucosal barrier in rats with CRC.Methods Sixty male Wistar rats were equally and randomly divided into five groups:normal control group (NC group),model group (MG group),low-dose PA group (PL group),middle-dose PA group (PM group),and high dose PA group (PH group) according to their body weight.The PL group,PM group,and PH group were given sodium phytate at 0.25,0.50 and 1.00 g/(kg · d),respectively,by gavage,while the NC group and MG group were given an equal volume of normal saline.From the 5th week to the 23rd week,all rats except those in the NC group were injected subcutaneously with dimethylhydrazine (30 mag/kg) in the back of the neck to induce CRC;the NC group received an equal volume of normal saline.All rats were sacrificed at the end of the 39th week.The colon tissue and the CRC tissue were collected from the rats in the NC group and the other groups,respectively,to measure the expression of zo 1 and occludin by immunohistochemistry and the expression of claudin-1 by Western blot.Results The incidence rates ofCRCin MGgroup,PL group,PM group,and PH group were 100％,100％,88.9％,and 66.7％,respectively.Compared with the NC group,the MG group had significantly lower expression of zo-1 and occludin (F =23.118,t=9.041;F=52.886,t=13.050;P＜O.05).Compared with the MG group,the PL group,PM group,and PH group had significantly higher expression of zo-1 and occludin (t =2.385-8.611,P＜0.05).Compared with the NC group,the MG group had significantly higher expression of claudin 1 (F=67.159,t =14.028,P＜0.05).Compared with the MG group,the PM group and PH group had significantly lower expression of claudin-1 (t =2.635,8.421;P＜0.05).Conclusion PA can improve the intestinal mucosal barrier by regulating the expression of zo-1,occludin,and claudim1to inhibit CRC.
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NSTL
万方数据
维普
