ROLE OF RAGE IN PATHOGENESIS OF PARKINSON'S DISEASE
Objective To investigate the role of receptor for advanced glycation end products (RAGE) in the pathogenesis of Parkinson's disease (PD).Methods A total of 30 male C57BL/6 mice were equally and randomly divided into three groups:normal control group,1-methyl-4-phenyl-1,2,3,6-tet rahydropyridine (MPTP) group,and MPTP+ RAGE-siRNA group.The MPTP group and the normal control group were injected intraperitoneally with MPTP and normal saline,respectively,for five consecutive weeks.The MPTP+RAGE-siRNA group was given RAGE-siRNA lentivirus in the substantia nigra by stereotaxic sur gery at one week before the treatment with MPTP which was the same as that for MPTP group.The expression of RAGE,tyrosine 3-monooxygenase (TH),and cyclooxygenase-2 (COX-2) in the substantia nigra of mice was measured by Western blot.Results Compared with the control group,the MPTP group had significantly higher levels of RAGE and COX-2 and a significantly lower levelofTH (F=62.25,q 14.92,P ＜0.01;F=17.83,q=8.15,P＜0.01;F=8.87,q=5.85,P＜0.01).Compared with the MPTP group,the MPTP+RAGE-siRNA group had significantly lower levels of RAGE and COX-2 and a significantly higher level ofTH (q=10.75,P＜0.01;q=5.47,P＜0.01;q=4.58,P＜0.05).Conclusion RAGE may be involved in the pathogenesisof PD through inflammatory mechanism.RAGE as a target for therapeutic intervention of PD ispromising in the future.
Parkinson diseaseglycosylation end products, advancedtyrosine 3 monooxygenaselentivirus
NETL
NSTL
万方数据
维普
