EFFECT OF CHEMOKINE CXCL1 ON EPITHELIAL-MESENCHYMAL TRANSITION OF BREAST CANCER MCF-7 CELLS
Objective To investigate the effects of chemokine (CXC motif) ligand 1 (CXCL1) on the migration and epithelial-mesenchymal transition (EMT) of human breast cancer cells (MCF-7) and its mechanism.Methods MCF-7 cells were cultured in vitro and divided into three groups:control group,CXCL1 (40 μg/L) group,and CXC chemokine receptor 2 (CXCR2) inhibitor (SB225002 10 mmol/L) group.Cell Counting Kit-8 (CCK8) was used to evaluate the proliferative activity of MCF-7 cells in all groups.Furthermore,enzyme-linked immunosorbent assay (ELISA) was used to measure the protein expression of E-cadherin,vimentin,p-PI3K,and p-AKT.The expression of E-cadherin mRNA and Vimentin mRNA was measured by RT-PCR.Results CCK8 assay results showed that compared with the control group,the CXCL1 group had a significantly higher cell proliferative activity (F=58.89,q =8.96,P ＜0.01),while the CXCR2 inhibitor group had a significantly lower cell proliferative activity (q =6.31,P＜0.05).The RT-PCR results indicated that the CXCL1 group had significantly lower expression of E-cadherin mRNA and significantly higher expression of Vimentin mRNA than the control group (t =4.07,3.62;P ＜ 0.05).The ELISA results showed that the CXCL1 group had significantly lower protein expression of E-cadherin and significantly higher protein expression of Vimentin (F =8.95,q =2.02,P ＜0.05;F =111.50,q =14.48,P ＜0.01);while the CXCR2 inhibitor group had significantly higher protein expression of E-cadherin and significantly lower protein expression of Vimentin (q =2.04,6.08;P ＜ 0.05).The CXCL1 group had significantly higher protein expression of p-PI3K and p-AKT than the control group (F =60.35,q =10.52,P＜0.01;F =28.15,q =5.37,P＜0.01);the CXCR2 inhibitor group had significantly lower protein expression of p-PI3K and p-AKT than the control group (q=4.65,4.97;P＜0.05).Conclusion Chemokine CXCL1 plays a key role in EMT of MCF-7 cells by activating the PI3K/AKT signaling pathway through CXCL1/CXCR2 axis.
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万方数据
维普
