DETECTION OF TUMOR ABNORMAL PROTEIN AND ITS CLINICAL SIGNIFICANCE IN CHILDREN WITH TUMOR
Objective To investigate the expression of tumor abnormal protein (TAP) and its correlation with the chemotherapeutic effect in children with acute lymphoblastic leukemia (ALL) or neuroblastoma (NB),and to provide a reference for the diagnosis,the evaluation of treatment effect,and monitoring of tumor recurrence in children with the two tumors.Methods A total of 93 children with tumor were enrolled in this study,including 67 ALL children and 26 NB children.Before treatment,TAP was detected by peripheral blood smear review in all patients;the serum level of lactate dehydrogenase (LDH) was measured in the sampled ALL children;the serum level of neuron-specific enolase (NSE) was measured in the sampled NB children.After complete remission,the serum level of LDH was measured and bone marrow biopsy was performed to detect minimal residual disease (MRD) in the sampled ALL children;the serum level of NSE was measured in the sampled NB children.Results The children with new-onset ALL had a significantly higher positive rate of TAP than the children with remission of ALL (62.50％ (20/32) vs 37.14％ (13/35);x2 =4.30,P＜0.05).There were no significant differences in the positive rate of TAP and the abnormal rate of LDH level between the children with new onset ALL and the children with remission of ALL (P＞0.06).For children with clinical complete remission of ALL,the positive rate of TAP in the MRD ＜10 4/L group was significantly lower than that in the MRD ≥10-4/L group (P=0.04).The children with new-onset NB had a significantly higher positive rate of TAP than the children with remission of NB (81.25％ (13/16) vs 30.00％ (3/10);P=0.02).There were no significant differences in the positive rate of TAP and the abnormal rate of NSE level between the children with new-onset NB and the children with remission of NB (P＞0.05).For children with new-onset NB,the positive rates of TAP in stage Ⅲ and Ⅳ were 75％ (6/8) and 87.50％ (7/8),respectively,and there was no significant difference in the positive rate of TAP between the children with new-onset NB in the two stages (P＞0.05).Conclusion TAP detection can be used as an indicator for screening out ALL and NB in children because of its high sensitivity,simple operation,and minimal invasion.The result of TAP detection is correlated with tumor burden,so it can be used for predicting recurrence after treatment.
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