NIS MUTATIONS IN PATIENTS WITH DIFFERENTIATED THYROID CANCER
Objective To determine the type and features of sodium/iodide symporter gene (NIS) mutations in patients with differentiated thyroid cancer (DTC),and to examine the relationship between the genotype and phenotype of these mutations.Methods Genomic DNA was extracted from the thyroid cancer tissues and normal thyroid tissues of 50 DTC patients.Mutations in all exons of the NIS gene were detected by PCR amplification and direct sequencing.Results A mutation (c.1571 C＞T,p.Leu 408=) was identified in exon 10 of the NIS gene in the tumor tissues of two patients;the mutant codon still encoded the same amino acid (leucine) as the wild-type.No mutation was found in normal tissues.There was no significant difference in the frequency of NIS mutations between tumor tissues and normal tissues (P＞0.05).Conclusion NIS mutations may not be associated with DTC in Qingdao,China,and the same-sense mutation detected in this study may not play a major role in iodine uptake in the thyroid.
NETL
NSTL
万方数据
维普
