INHIBITORY EFFECT OF LONG NON-CODING RNA NEAT1 ON HEPATOMA CELL APOPTOSIS
Objective To investigate the effect of long non-coding RNA NEAT1 (lncRNA NEAT1) on hepatoma cell apoptosis.Methods lncRNA NEAT1 expression in cancer tissues before and after transcatheter arterial chemoembolization (TACE),in normal liver LO2 cells,and in hepatoma cell lines (SMMC-7721,Hep3B,Hub7,and HepG2) were measured by quantitative real-time PCR.The lncRNA NEAT1 siRNA was transfected into HepG2 cells using LipofectamineTM 2000 to inhibit lncRNA NEAT1 expression.Cell viability was measured by MTT assay.Caspase-3 activity was measured using the caspase-3 activity kit.Bax and Bcl-2 protein expression was determined by Western blot.Results lncRNA NEAT1 expression was significantly downregulated after TACE treatment (t =6.26,P＜0.05).In addition,lncRNA NEAT1 expression was significantly upregulated in SMMC-7721,Hep3B,Hub7,and HepG2 cells than in normal liver LO2 cells (F =30.02,P＜0.05).Compared with the negative control (NC) group,HepG2 cells treated with lncRNA NEAT1 siRNA had significantly reduced viability (F=15.97,P＜0.05),enhanced caspase-3 activity (F=28.36,P＜0.05),upregulated Bax protein level (F =9.85,P＜0.05),and downregulated Bcl-2 protein level (F=6.73,P＜0.05).Conclusion lncRNA NEAT1 not only inhibits hepatoma cell viability,but it may also be a new biomarker for the development and progression of hepatocellular carcinoma (HCC) and a new target for HCC prevention and treatment.
RNA, long noncodingcarcinoma, hepatocellularapoptosis
NETL
NSTL
万方数据
维普
