EFFECT OF RECOMBINANT HUMAN GROWTH HORMONE ON INFLAMMATORY FACTORS AND IMMUNE AND RENAL FUNCTION IN MICE WITH ACUTE KIDNEY INJURY INDUCED BY SEPSIS
Objective To investigate the effect of recombinant human growth hormone (rhGH) on renal function,immune function,and inflammatory factors in mice with acute kidney injury (AKI) induced by sepsis.Methods A total of 75 specific pathogen-free C57BL/6 mice were randomly divided into groups A,B,and C.In group A,the abdominal cavity was opened and then closed without special treatment;in group B,cecal ligation and puncture was performed to establish a mouse model of sepsis;in group C,intraperitoneal injection of rhGH was performed in addition to the treatment for group B.The mice were decapitated at 48 hours and related samples were collected.ELISA was used to observe the changes in the serum levels of immunoglobulin A (IgA),IgM,and IgG,as well as the levels of serum creatinine (SCr),blood urea nitrogen (BUN),and urinary kidney injury molecule-1 (KIM-1).Western blot was used to measure the expression of interleukin-18 (IL-18),macrophage marker CD68,and matrix metalloproteinase-2 (MMP-2) in renal tissue.HE staining was used to observe the pathological changes of the kidney.Results Compared with group A,group B had significant reductions in the serum levels of IgA,IgM,and IgG (t =22.8-240.0,P＜0.05),significant increases in the levels of IL-18,CD68,and MMP-2 in primary renal cells (t =20.5-26.6,P＜0.05),and signifi cant reductions in the levels of SCr,BUN,and KIM-1 after surgery (t =18.6-32.8,P ＜0.05).Compared with group B,group C had significant increases in the serum levels of IgA,IgM,and IgG (t =19.3-261.2,P ＜0.05),significant reductions in the levels of IL-18,CD68,and MMP-2 in primary renal cells (t =30.0-40.0,P ＜0.05),and significant increases in the serum levels of SCr,BUN,and KIM-1 after surgery (t =29.6-59.1,P＜0.05).Compared with group A,group B had varying degrees of kidney injury;compared with group B,group C had significant reductions in kidney injury and the degree of renal interstitial congestion.Conclusion In AKI mice,rhGH may enhance immune function,alleviate inflammatory response,maintain normal metabolic function of the kidney,and thus exert its protective effect against AKI induced by sepsis.
human growth hormonesepsisacute kidney injuryinterleukin-18immunoglobulins
NETL
NSTL
万方数据
维普
