Effect of overexpression of SQLE on the proliferation,migration,and invasion ability of cervical cancer Hela cells
Objective To investigate the expression of squalene epoxidase(SQLE)in cervical cancer Hela cells and its effect on the proliferation,migration and invasion of cervical cancer cells and its potential mechanism.Methods Hela and H8 cells were sellected for this research,SQLE overexpression stably transfected cell lines were constructed by lentivir-us,the experiment was divided into H8 group,Hela group and Hela-SQLE group.Cell proliferation was detected by CCK8 assay.The mRNA expressions of SQLE,Vimentin,E-cadherin,and α-SMA were detected by qRT-PCR.Cell mi-gration and invasion were detected by Transwelll.Western blotting was used to detect the protein expression of SQLE,Vimentin,E-cadherin,and α-SMA.Results The results of CCK8 experiment showed that the proliferation rate of H8 group,Hela group and Hela-SQLE group was statistically significant at 24,48 and 72 h,with F values of 80.450,140.900 and 430.100,and P values of 0.003,0.006 and＜0.001,respectively.The results of Transwell migration ex-periment showed that the number of H8 cells,Hela cells and SQLE overexpressed Hela cells migrated was(28.43± 5.22),(100.43±5.56)and(139.29±7.61),H=17.840,P＜0.0001.Transwell invasion assay showed that the number of H8 cells,Hela cells and SQLE overexpressed Hela cells invaded was(30.43±1.99),(92.43±9.29)and(129.29±11.80),H=17.850,P＜0.0001.qRT-PCR results showed that the relative expression levels of SQLE mRNA in H8 cells,Hela cells and SQLE overexpressed Hela cells were 1.00±0.14,6.32±0.83 and 153.17±10.27,H=7.200,P=0.004;The relative expression levels of Viementin mRNA were 1.00±0.10、6.94±0.53 and 12.32±0.84,H=7.261,P=0.004;The relative expression levels of α-SMA mRNA were 1.00±0.18、3.39±0.40 and 5.77±0.34,H=7.261,P=0.004;The relative expression levels of E-cadherin mRNA were 1.00±0.07,0.07±0.01 and 0.03±0.00,H=7.261,P=0.004.Western blot results showed that the relative expression levels of SQLE protein in H8 cells,Hela cells and SQLE overexpressed Hela cells were 0.15±0.03,0.45±0.02 and 0.64±0.05,H=4.571,P=0.0002;The relative expression levels of Vie-mentin protein were 0.08±0.03,0.19±0.02 and 0.35±0.03,F=54.220,P=0.006;The relative expression levels of α-SMA protein were 0.06±0.01,0.17±0.00 and 0.45±0.01,F=948.000,P=0.005;The relative expression levels of E-cadherin protein were 0.41±0.01,0.20±0.04 and 0.08±0.00,F=115.800,P=0.033.Conclusions The expression of SQLE in cervical cancer cells is increased.SQLE overexpression promotes the proliferation,invasion and migration of cervical cancer cells.SQLE may be a potential cervical cancer promoting factor.
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