Relationship between interleukin-6 and the markers of inflammatory response and immune cell infiltration in colorectal cancer and its impact on prognosis
Objective To investigate the relationship between interleukin-6(IL-6)and the markers of inflammatory response in colorectal cancer and its effect on immune cell infiltration,and to analyze its role in prognostic stratification.Methods The clinicopathological data of 167 patients diagnosed with colorectal cancer from April 1,2021 to June 30,2023 in the Shandong Provincial Hospital Affiliated to Shandong First Medical University were retrospectively collected.The cut-off values of platelet-lymphocyte ratio(PLR),neutrophil-lymphocyte ratio(NLR),lymphocyte-monocyte ratio(LMR)and systemic immune inflammatory index(SII)were obtained from the receiver operating characteristic curve,and were divid-ed into two groups.test or Fisher exact probability method and logistic regression were used to analyze the relationship between IL-6 and clinicopathological characteristics and inflammatory response markers in colorectal cancer patients.The relationship between IL-6 expression and immune cell infiltration in colorectal cancer tissue was analyzed u-sing the Tumor Immunology and Microenvironment Resource(TIMER)2.0 database.The expression levels of IL-6 in ad-jacent normal tissue,primary colorectal cancer lesions,and metastatic lesions of colorectal cancer patients,as well as the relationship between IL-6 and prognosis in colorectal cancer and rectal cancer patients,were analyzed using the Kaplan-Meier Plotter database.Results The high expression of IL-6 in colorectal cancer was associated with the high expression of multiple cytokines(IL-1β,IL-2,IL-5,α interferon,IL-8 and IL-12P70),and was associated with low PLR,low LMR,high NLR and high SII(all P＜0.05).High expression of IL-6 was positively correlated with CD8+T cell,neu-trophil and dendritic cell infiltration in colorectal cancer(all P＜0.001).High IL-6 in colon cancer patients was signifi-cantly associated with poor overall survival rate(P=0.001)and boardline associated with poor recurrence-free survival rate(P=0.053).IL-6 expression in rectal cancer was not associated with overall survival rate and recurrence-free surviv-alrate(both P＞0.05).Conclusion High expression of IL-6 is associated with colorectal cancer staging,multiple inflam-matory cytokines,immune cell invasion and poor prognosis of colon cancer,which suggests that IL-6 has potential clinical value in monitoring the course of colorectal cancer,evaluating inflammation and immune status,and predicting survival.
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