Single cell protein technology and its application in cancer
Objective To review the emerging technologies in single-cell proteomics and their different applications in vari-ous types of cancer.Methods By using the PubMed and China National Knowledge Infrastructure databases,relevant lit-erature from January 1,2006 to May 1,2023 were searched with Chinese and English keywords of"single cell,proteomics,mass spectrometry,cancer".Inclusion criteria included:(1)The overview of single-cell omics;(2)Overview of single-cell proteomics in single-cell omics;(3)Single-cell omics mass spectrometry;(4)Single-cell proteomics in cancer.Exclusion criteria was informal publications such as conference abstracts and Chinese disertations.A total of 37 articles were ulti-mately included.Results Single-cell proteomics has seen emerging technologies in many areas,particularly related to sam-ple preparation and separation,electrolysis and ionization,mass spectrometry,and electrical separation.Single-cell pro-teomics has promising applications for cancer surveillance,immunotherapy,targeted therapy,radiotherapy,chemotherapy,etc.,and it can also be used for prognostic evaluation of cancer.However,there are still some technical challenges,such as sensitivity and efficiency.Conclusion Despite certain progress in cancer surveillance,immunotherapy,targeted therapy,ra-diotherapy,chemotherapy and prognosis has been made,Single-cell proteomics is still challenged by issues related to sensi-tivity,efficiency and generalizability.
single cellproteomicsmass spectrometrycancerreview literature
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