Chemical composition analysis of Hemerocallis hybrida Bergmans based on UHPLC-Q-TOF-MS/MS technology
Objective By adopting ultra high performance liquid chromatography cascade Quadrupole time-of-flight mass spectrometry to analyze the chemical composition of Hemerocallis hybrida Bergmans.Methods The gradient elution was carried out on A Waters HSS T3 column with 0.1％formic acid acetonitrile(A)-0.1％formic acid water(B)as the mobile phase in negative ion mode.The flow rate was 0.4 ml/min,the column temperature was 35,and the injection volume was 4 pl.The chemical composition database of Hemerocallis was established,and the compounds were identified by accurate m/z values,characteristic secondary data,a variety of databases and cleavage pathways in the literature.Results A total of 59 compounds were identified,including 25 flavonoids,22 phenylpropanoids,4 fatty acids,2 alkaloids and 6 other compounds.Among them,12 compounds were first reported in Hemerocallis.Conclusions This method is the first time to systematically,comprehensively and quickly screen and identify the compounds in Hemerocallis hybrida Bergmans,which provides a basis for the development and in-depth study of Hemerocallis hybrida Bergmans.
Hemerocallis hybrida BergmansUltra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometryChemical composition
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