Expression and significance of MIOX in renal cell carcinoma tissues
Objective To investigate the effect of myoinositol oxygenase(MIOX)on the proliferation,invasion,and metastatic ability of renal cancer cells and to explore its role in renal cancer.Methods The TCGA database was used to analyze the expression of MIOX in human renal cancer tissues and normal renal tissues;TIMER 2.0 was used to analyze the expression of MIOX in tumor tissues and normal tissues of different sites.The HPA database was used to collect protein expression data;GEPIA database analysis was used to explore the effect of MIOX on the prognosis of renal cancer;the LinkOmics database was used to find the co-expressed genes of MIOX and perform GO enrichment analysis and KEGG pathway analysis;RT-qPCR was used to detect the expression level of MIOX mRNA in renal cancer cells and tissues.Overexpression plasmid regulating MIOX expression in cells was followed by cell scratch assay,Transwell assay,and CCK8 assay to detect the effects on cell proliferation,metastasis,and invasion ability after regulating MIOX.Results The mRNA and protein levels of MIOX in renal cancer were significantly lower in cell lines and tissues than in normal cells and tissues(P<0.05).Renal cancer patients with high expression of MIOX had better DFS and OS than those with low expression of MIOX(P<0.05).Overexpression of MIOX inhibited the proliferation,invasion,and metastatic ability of A498 cells and CAKI-1 cells(P<0.05).Conclusions MIOX was low-expressed in renal cancer tissues and cells,and its low-expression patients had a poorer prognosis.Overexpression of MIOX can inhibit the proliferation,migration,and invasion of renal cancer cells,and MIOX is expected to be a new biomarker and therapeutic target for renal cancer.
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