Value of basic information analysis and related detection methods in clinical diagnosis of pulmonary tuberculosis in Qinglong County,Guizhou Province
Objective To analyze the basic information of pulmonary tuberculosis patients who visited Qinglong County People's Hospital in Guizhou Province from January 2022 to October 2023,providing a basis for the prevention and control of tuberculosis,and to explore the application value of three detection methods-Ziehl-Neelsen acid-fast stain,Roche culture,and Mycobacterium tuberculosis complex nucleic acid detection-in the diagnosis of pulmonary tuberculosis in grassroots hospitals.Methods A retrospective analysis was conducted on the clinical data of 306 suspected pulmonary tuberculosis patients who visited the tuberculosis clinic at Qinglong County People's Hospital from January 2022 to October 2023.Based on clinical diagnosis,the subjects were divided into two groups:the pulmonary tuberculosis group(209 cases)and the non-tuberculosis group(97 cases).The epidemiological characteristics of the 209 pulmonary tuberculosis patients were analyzed,and the results of acid-fast staining,Roche culture,and nucleic acid detection in both groups were analyzed to evaluate the application value of different combined detection methods in pulmonary tuberculosis.Results The male-to-female ratio in the 209 pulmonary tuberculosis patients was close to 3:1(P<0.05);the age of onset was mainly concentrated in the 15～65 age group,higher than other age groups;the general education level of the patients was not high,with the proportion of primary school to junior high school education being higher than that of bachelor's degree or above(P<0.05);the proportion of farmers and migrant workers was higher than other occupations(P<0.05),but the proportion of students has been increasing in recent years,accounting for 14.83%;the prevalence of tuberculosis was higher in patients with a smoking habit(P<0.05),and the prevalence of tuberculosis in patients with malnutrition was higher than in those with normal nutritional status(P<0.05);most patients with tuberculosis had a history of underlying diseases(P<0.05)and a history of tuberculosis and contact with tuberculosis(P<0.05).The positive rate of acid-fast staining plus nucleic acid detection was 89.00%,which was higher than that of single use of the three methods and acid-fast staining plus Roche culture(P<0.05).The sensitivity and specificity of acid-fast staining,Roche culture,and nucleic acid detection were 29.67%,45.93%,and 80.38%,and 87.6%,91.75%,and 62.89%,respectively.The sensitivity of acid-fast staining plus Roche culture and acid-fast staining plus nucleic acid detection were 70.33%and 89.0%,respectively;and the specificity were 97.94%and 84.53%,respectively.The sensitivity of acid-fast staining plus nucleic acid detection was higher than that of acid-fast staining plus Roche culture and single detection(acid-fast staining,Roche culture,nucleic acid detection)(P<0.05).The specificity of acid-fast staining plus Roche culture assay was higher than that of acid-fast staining plus nucleic acid assay and single assay(acid-fast staining,Roche culture,nucleic acid assay)(P<0.05).The results of ROC curve analysis showed that the combined detection of acid-fast staining and nucleic acid was more effective than other methods in the diagnosis of tuberculosis.Conclusions According to the preliminary investigation and statistics of the basic information of the patients,the distribution of tuberculosis patients was diversified,and early diagnosis was difficult.The People's Hospital of Qinglong County,the only designated tuberculosis hospital in the region,was assessed for the diagnostic value of the combined tests based on the comparison of positive test methods.The rational and effective use of combined detection in the laboratory is of high clinical value in the diagnosis and treatment of tuberculosis.
万方数据
维普
