四指马鲅热休克蛋白70基因(HSP70)表达分析
Expression Analysis of Heat Shock Protein 70(HSP70)Gene in Eleutheronema tetradactylum
吴子宜 1赖文杰 2邹振江 2宋亚康 2黄舜梅 3汤胜亮 3卢丹琪 1张勇1
作者信息
- 1. 中山大学生命科学学院,广州 510275;中山大学广东省水生经济动物良种繁育重点实验室,广州 510275;南方海洋科学与工程广东省实验室(珠海),广东珠海 519000
- 2. 中山大学生命科学学院,广州 510275;中山大学广东省水生经济动物良种繁育重点实验室,广州 510275
- 3. 珠海市龙胜良种鱼苗培育有限公司,广东珠海 519090
- 折叠
摘要
四指马鲅(Eleutheronema tetradactylum)为名贵海产鱼类,因其对应激反应强烈,易在运输过程中受损.热休克蛋白 70(heat shock protein 70,HSP70)作为一种热休克蛋白(heat shock protein,HSP),是 HSP 家族中最重要的一员,对细胞蛋白质折叠过程及细胞对压力的适应起着重要作用.比较四指马鲅仔鱼和幼鱼在运输胁迫下的抗氧化能力和应激反应,进行幼鱼鳃组织学观察,探究热休克蛋白HSP70基因的组织表达模式以及在仔鱼和幼鱼中的表达差异,研究验证了运输胁迫下仔鱼和幼鱼阶段四指马鲅的抗氧化能力和HSP70表达呈现显著差异,并探讨了其作为评估抗应激反应及养殖管理效果的生物指标的潜力.
Abstract
Eleutheronema tetradactylum is a valuable marine fish,which is susceptible to damage during transportation due to its strong response to stress.As a heat shock protein(HSP),HSP70,the most important member of the HSP family,plays a crucial role in cellular protein folding and cellular adaptation to stress.By comparing the antioxidant capacity and stress response of the larval and juvenile fish under transportation stress,histological observations of juvenile gill tissues were conducted to explore the tissue expression patterns of the heat shock protein HSP70 gene and the expression differ-ences between larvae and juveniles.Significant differences in antioxidant capacity and HSP70 expression between larval and juvenile Eleutheronema tetradactylum under transportation stress were confirmed,thus highlighting the potential of HSP70 ex-pression as biological indicators for assessing stress response and aquaculture management effectiveness.
关键词
四指马鲅/运输胁迫/抗氧化能力/HSP70/表达分析Key words
Eleutheronema tetradactylum/transportation stress/antioxidant capacity/HSP70/expression analysis引用本文复制引用
基金项目
广东省科技计划(2023B1212060023)
南方海洋科学与工程广东省实验室(珠海)科研项目(SML2023SP201)
出版年
2024
国家科技期刊平台
NSTL
万方数据