台湾泥鳅腐皮病霍乱弧菌的分离与鉴定
Isolation and Identification of V.cholerae from the Rotting Skin Disease of Paramisgurnus dabryanus ssp.
杨宁 1姜芳燕 2陈攀 1马军 1陈燕 1曹柳 1侯兴蓉1
作者信息
- 1. 海南热带海洋学院热带海洋生物资源利用与保护教育部重点实验室,海南三亚 572022;海南热带海洋学院海南省热带海洋渔业资源保护与利用重点实验室,海南三亚 572022;海南热带海洋学院海南热带海洋学院崖州湾创新研究院,海南三亚 572022
- 2. 琼台师范学院热带生物多样性与资源利用实验室,海口 571127
- 折叠
摘要
为研究台湾泥鳅(Paramisgumus dabryanus ssp.)腐皮病的病原,对其腐皮等病变部位进行细菌分离纯化,获得1株优势菌株,命名为P1,利用革兰氏染色观察其形态特征,利用PCR扩增技术、16SrRNA基因测序,得到病原菌的序列,用MEGA软件构建系统进化树,再结合生理生化分析结果鉴定菌种名称.结果发现:革兰氏染色阴性,弧形,两端钝圆;菌体长1～1.5 μm,宽度1～1.2 μm,有菌丝和鞭毛,16S rRNA基因1 395 bp.建立系统进化树后发现,P1与霍乱弧菌处于同一分支;生理生化实验结果与霍乱弧菌一致,从而鉴定该菌株为霍乱弧菌(Vibrio cholerae).药敏实验结果表明,该分离菌株对头孢哌酮、卡那霉菌、万古霉素等10种抗生素高度敏感;对四环素中度敏感;对呋喃唑酮、复方新诺明、克林霉素等17种抗生素耐药.实验结果为台湾泥鳅腐皮病的病原鉴定与药物防治提供参考.
Abstract
In order to study the pathogen of P.dabryanus ssp.,a dominant strain named P1 was obtained by bacterial isolation and purification of its rotting skin and other lesions.The morphological characteristics of the strain were observed by Gram staining,and the sequence of the pathogen was obtained by PCR amplification and 16S rRNA gene sequencing.The phylogenetic tree was constructed by MEGA software.Then the name of the bacterial species was identified through physiological and biochemical analysis.The results showed that Gram staining was negative,curved,and bluntly rounded at both ends;the bacterium was 1-1.5 μm in length,1-1.2 μm in width,with mycelium and flagellum;16S rRNA gene was 1 395 bp;the establishment of the phylogenetic tree revealed that P1 was in the same branch with Vibrio cholerae;the results of physiological and biochemical experi-ments were in agreement with those of Vibrio cholerae,which leads to the identification of the strain as Vibrio cholerae.The results of drug sensitivity test showed that the isolated strain was highly sensitive to nine antibiot-ics,including cefoperazone,canomyces and vancomycin,moderate sensitive to tetracycline,resistant to 18 antibiot-ics,including furazolidone,cotrimoxazole and clindamycin.The results provide reference for the pathogen identifi-cation and drug control of P.dabryanus ssp..
关键词
台湾泥鳅/腐皮病/霍乱弧菌/分离/鉴定Key words
Paramisgurnus dabryanus ssp./rotting skin disease/Vibrio cholerae/isolation/identification引用本文复制引用
基金项目
三亚市院地科技合作项目(2016YD20)
海南热带海洋学院科研启动基金(RHDRC202101)
海南省院士创新平台资金项目()
出版年
2024
国家科技期刊平台
NSTL
万方数据