Value of Helicobacter pylori resistance gene detection in clinical precision therapy
Objective To investigate the infection status of Helicobacter pylori(Hp)in patients with stomach discom-fort in Xuancheng Central Hospital,and evaluate the accuracy of fecal sample detection for Hp infection and the preci-sion treatment effect after drug resistance gene testing combined with fecal Hp nucleic acid and drug resistance gene tests.Methods Patients with stomach discomfort admitted to the Gastroenterology Department in a hospital were in-cluded from January to December 2023.All patients underwent screening with carbon-13 urea breath test(13C-UBT)and rapid urea test(RUT).Then the patients with double positive or double negative findings were included as study sub-jects,and undergone fecal Hp DNA detection.The consistency between the findings of fecal Hp detection and 13C-UBT+RUT detection was analyzed.Patients with positive fecal Hp and with voluntary intention to receive resistance gene test-ing(experimental group)were given precision treatment based on the test results,and Hp infected victims who did not undergo resistance gene testing(control group)were treated with conventional quadruple therapy regimen.Finally,the clinical prognosis was compared between the experimental group and the control group.Results Among the 446 pa-tients included in the study,170 were detected as Hp double positive by 13C-UBT+RUT,and 162 were detected as Hp positive by 13C-UBT+RUT and fecal Hp DNA detection.The consistency between fecal Hp DNA detection and 13C-UBT+RUT detection was good in general(Kappa value=0.962,P＜0.05).Seventy-three patients were finally included in the experimental group,and 89 in the control group.The detection rate of mutant strains in the experimental group was 73.97％.The clinical curative rate was 90.41％(66/73)in the experimental group,which was higher than that in the con-trol group 67.42％(60/89).The difference was significant(x3=12.270,P＜0.05).Conclusion The results of fecal Hp DNA detection are accurate and reliable,and can be used for bacterial resistance detection.This test protocol can be guiding value for precision medication in clinic,and conducive to improving the clinical curative rate for the patients.
万方数据
维普
