Copulation,embryonic and post-embryonic development of Sphaeramia nematoptera
The copulation behavior,embryonic and post-embryonic development of Sphaeramia nematoptera were investigated in this study.The results showed that the mating behavior was dominated by females,mating between 1:00 and 3:00 am,and the reproductive cycle was 11~18 d without seasonal fluctuations.The release of eggs by female were 3126~4882 at a time,which were incubated by the male's mouth.The fertilized eggs were suborbicular,(720±10)μm in diameter,with several oil globules inside.It took 187 h for a fertilized egg to be hatched under(27.0±0.5)℃.The embryo development process included Cleavage,Blastocyst,Gastrulation,Neurula and Organ formation stages.The post-embryonic development could be divided into juvenile stage,larval stage,juvenile stage and adult stage.The whole length of the first hatchling was(2.8±0.1)mm,the height of the mouth cleft was(0.202±1 8)mm,and the juvenile opened its mouth to feed at the second day after hatching.Fins began to form and two black bands appeared on the body when ending the floating stage and entering the larval stage 24d after hatching.Scales were fully formed 50d after hatching and the body color and markings as well as other appearance characteristics were similar to the adult fish,which indicated that the fish entered the juvenile stage.Maturation of gonad was achieved 360d after hatching.
Sphaeramia nematopteramarine ornamental fishreproductive habitsembryonic developmentpost-embryonic development