Study on the chemical constituents and pharmacological activity of the marine-derived fungus Aspergillus fumigatus DL-p0m-g2 in the Beibu Gulf
The chemical constituents and their bioactivities of Aspergillus fumigatus DL-p0m-g2 from the Beibu Gulf were studied in order to obtain bioactive compounds.The compounds were isolated and purified by reversed-phase ODS column chromatography,semi-preparative liquid chromatography and other chromatographic methods.Their structures were identified by physicochemical properties,mass spectrometry and nuclear magnetic resonance spectroscopy.The cytotoxic,antibacterial and cholesterol transporter NPC1L1 protein binding activities of the isolated compounds were evaluated.As a result,a total of 21 alkaloids and one steroid were isolated and identified as 6-methoxyspirotryprostatin B(1),spirotryprostatin A(2),fumitremorgin C(3),cyclotryprostatin A(4),fumitremorgin B(5),pseurotin A(6),azaspirofuran A(7),azaspirofuran B(8),cephalimysin C(9),cephalimysin B(10),fumiquinazoline C(11),fumiquinazoline B(12),fumiquinazoline A(13),fumiquinazoline D(14),fumiquinazoline F(15),tryprostatin B(16),verruculogen(17),chaetominine(18),bisdethiobis(methylthio)glitoxin(19),helvolic acid(20),7-deacetylpyripyropene A(21),terezine D(22).Compound 6 showed moderate cytotoxic activity against human hepatoma cells(HepG2),human lung cancer cells(A549)and human rectal cancer cells(HCT116).Compounds 1,3 and 20 showed antibacterial activity against Staphylococcus aureus.Compound 14 showed a good binding with NPC1L1 protein,indicating its potential in the development of lipid-lowering drugs.
Aspergillus fumigatuschemical constituentscytotoxic activityanti-bacteria activityNPC1L1 protein binding
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