Study on the community structure of eukaryotic phytoplankton in the Shenzhen Bay based on high-throughput sequencing technology
Using the 18S rDNA V4 region as the target gene,Illumina Miseq high-throughput sequencing technology was applied to study the phytoplankton community in the seawater of the Shenzhen Bay during the four seasons from the winter of 2019 to the spring of 2021,and to compare with the historical data of harmful algal blooms species in this area,in order to explore the community composition and succession pattern of toxic and harmful phytoplankton in the Shenzhen Bay.The high-throughput sequencing results showed that the eukaryotic phytoplankton in the sea waters of the Shenzhen Bay consisted of 7 groups,including Bacillariophyta,Dinoflagellata,Chlorophyta,Ochrophyta,Haptophyta,Cryptophyta,and Rhodophyta,with a total of 82 families and 269 species.Among them,microphytoplankton was the main group,Nannochloris sp.was the dominant species in spring and autumn,Isochrysis galbana was the dominant species in summer,and Thalassiosira minima was the dominant species in winter.The phytoplankton diversity index in winter was the highest in the four seasons.A total of 32 species of toxic and harmful phytoplankton were detected in this survey,including 15 dinoflagellata species,13 bacillariophyta species,3 ochrophyta species,and 1 of haptophyta species.Compared with the historical data,the toxic and harmful phytoplankton in the Shenzhen Bay showed a miniaturization trend and an increasing diversity.The monophasic harmful blooms dominated by dinoflagellates or diatoms have been succeeded to polyphasic blooms of dinoflagellates and diatoms.
Shenzhen Bayeukaryotic phytoplanktontoxic and harmful algaehigh-throughput sequencing
万方数据
维普
