Spermary development,spermatogenesis and sperm ultrastructure of Paphia textile in the Beibu Gulf,Guangxi
In this study,anatomical observation,spermary tissue section,scanning and transmission electron microscopy techniques were used to study the spermary development,spermatogenesis and ultrastructure of mature sperm of Paphia textile in Beibu Gulf of Guangxi.The results showed that no hermaphrodites were found in the collected samples,and all the observed individuals were dioecious.The spermary development of P.textile was divided into five main stages:proliferating stage,growing stage,maturing stage,spawning stage,and resting stage.The maturing stage and spawning stage of P.textile was from September to January of the following year.Sperm development is divided into five stages:spermatogonia stage,primary spermatocyte stage,secondary spermatocyte stage,spermatid stage and mature sperm stage.The mature sperm of P.textile consisting of the head,middle and tail,overall length approximately 46.04 μm.The sperm head is divided into the acrosome and the nucleus.The acrosome is conical in shape and the nucleus is long and cylindrical.The anterior end was 0.71 μm in diameter and the posterior end was about 1.34 μm in diameter.The middle part consisted of four mitochondria encircled in a circular shape and two mutually perpendicular proximal and distal centrioles.The tail flagellum was about 38.8 μm long and 0.21 μm in diameter,and the external plasma membrane was covered with a typical"9 + 2"duplex microtubule structure.The results of this study provide basic research information for the reproductive biology,artificial seed breeding and germplasm resource conservation of P.textile.
万方数据
维普
