Complete mitochondrial genome of Cladopsammia gracilis and Rhizopsammia wettsteini(Scleractinia,Dendrophylliidae)and its phylogenetic implications
There is a taxonomic uncertainty on the species classification of Cladopsammia gracilis and Rhizopsammia wettsteini as well as the unresolved status of the closely related genera Cladopsammia and Rhizopsammia.In order to further analyze the phylogenetic relationship between C.gracilis and R.wettsteini,we examined the micromorphological and microstructural features and systematically compared the gene structure,genetic distance and codon preference of two mitochondrial genomes.The results show that there are three distinct characteristics:reptoid budding,costae and septa.The genome composition of the two species is very similar,containing 13 protein-coding genes,2 rRNA genes and 2 tRNA genes.Unlike most scleractinians,there were no overlaps between genes.In addition,phylogenetic analysis based on CO1 genes show that C.gracilis and R.wettsteini are closely related but distantly related to other species of the genera Cladopsammia.The present study highlights the need for an integrative approach to conduct a deeper species delimitation of genera Cladopsammia and Rhizopsammia.