首页|河口岸带氨氮原位快速分析方法研究

河口岸带氨氮原位快速分析方法研究

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以自主研发的海水氨氮原位快速分析仪的现场可靠测量为目标,基于改进的靛酚蓝分光光度法建立了一种河口岸带氨氮的快速分析方法.论文基于正交实验分析,研究了不同盐度条件下,全自动快速测量最佳不完全显色反应时间,并确定了给定盐度范围内最佳不完全显色反应时间随盐度变化的规律.研究及分析结果表明,样品盐度为 5‰的情况下,基于改进和优化后的靛酚蓝分光光度法,氨氮原位测量仪的检测耗时仅需要 60s左右;样品盐度在 0‰～25‰范围内,氨氮快速测量的最佳反应时间,随着盐度的增大而逐渐增加,样品盐度为 25‰时,检测耗时为 180s.光程长度为 10cm、显色反应时间为 60s时,盐度为 5‰的氨氮样品检测下限为 0.9945μmol·L-1,方法标准工作曲线R2大于 0.99,证明改进后的靛酚蓝分光光度法可以满足河口岸带水体中氨氮的原位快速检测.将其应用于湛江湾水体中氨氮时间序列在线监测发现,监测期间氨氮浓度与水体盐度存在很好的负相关关系.与传统的人工检测和流动注射分析方法相比,自主研发的海水氨氮原位快速分析仪检测分析过程中样品和试剂消耗量极少(μL),反应时间短(盐度为 5‰时 60 s),可适用于河口岸带水体中氨氮的长时间序列原位及在线监测.
In situ rapid analytical method for ammonia nitrogen concentration in estuarine zone
With the goal of in situ reliable measuring of the ammonia nitrogen by a self-developed in situ analyzer,a rapid analysis method for ammonia nitrogen concentration in estuarine zones has been established based on the improved indophenol blue spectrophotometry.Through the orthogonal experimental analysis on the incomplete chromogenic reaction time and salinity,the optimal incomplete chromogenic reaction time for in situ automated rapid measurements under different salinity conditions has been established and the variation of the optimal incomplete chromogenic reaction time of the samples with salinity in each range has been determined.The study shows that the optimal reaction time for the rapid measurement of ammonia nitrogen in the range of salinity from 0‰ to 25‰ gradually increases with the increase of salinity and the minimum reaction time is 60s(salinity:5‰)and the maximum reaction time is 180s(salinity:25‰).With a 10 cm LWCC(liquid waveguide capillary cell)and 60s reaction time,the detection limit of this method acquired by taking 11 parallel samples of blank samples is 0.9945 μmol·L-1(salinity:5‰),and the R2 of the standard working curve of the method was more than 0.99,which proved that the improved indophenol blue spectrophotometric method can meet the in situ rapid analysis of ammonia nitrogen in estuarine zones.The in situ analyzer has deployed in the Zhanjiang Bay for the time series monitoring of ammonia nitrogen concentration,and the monitoring data revealed that there is a good negative correlation between the ammonia nitrogen concentration and the salinity in the Zhanjiang Bay during the monitoring period.The consumption of samples and reagents during the detection and analysis process is very small(μL),and the reaction time is short(60 s,salinity:5‰),and these features make the analyzer ideally suited for long time series monitoring of ammonia nitrogen concentration in estuarine waters.

ammonia nitrogensequential injection analysisincomplete color renderingrapid measurementspectrophotometry

和嘉伟、杨泽明、赵金成、张现清、李彩、李智豪、林勇全、陈容

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热带海洋国家重点实验室,中国科学院南海海洋研究所,广东广州 510301

中国科学院大学,北京 100049

广州和时通电子科技有限公司,广东 广州 510300

氨氮 顺序注射分析 不完全显色 快速测量 分光光度法

广东省重点领域研发项目广州市南沙区科技规划项目国家重点研发计划国家重点研发计划广东省基础与应用基础研究基金海南省自然科学基金热带海洋学国家重点实验室开放课题计划

2020B11110200042022ZD0012016YFC14006032017YFC05063052021A1515110639422QN441LTOZZ2003

2024

10.11978/2023144

热带海洋学报
中国科学院南海海洋研究所

热带海洋学报

CSTPCD北大核心
影响因子：0.513
ISSN：1009-5470
年,卷(期)：2024.43(4)
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