Study on secondary metabolites of the endophytic fungus Talaromyces sp.XXH006 Isolated from Conus literatus
To investigate the chemical constituents and bioactivities of secondary metabolites extracted from Talaromyces sp.,which was isolated from Conus literatus.Various of separate materials and technologies such as silica gel,reverse phase silica gel,sephadex LH-20,MCI and semi-preparative high performance liquid chromatography were employed to obtain pure compounds.These compounds were then identified by nuclear magnetic resonance,mass spectrometry etc.Subsequently,bioactivities of fourteen compounds were assessed using the CCK8 assay to explore the inhibition against tumor cell line U87 and A549.These compounds,expect compound 5 and 6 identified for the first time in Talaromyces sp.,included ergosteryl stearate(1),ergosterol(2),β-sitosterol(3),2-hydroxyemodin(4),(+)-rugulosin(5),1,1′,3,3′,5,5′-hexahydroxy-7,7′-dimethyl[2,2′-bianth-racene]-9,9′,10,10′-tetrone(6),rel-(6R,7R)-5,6,7,8-tetrahydro-6-hydroxy-7-methyl-8-oxo-3-propyl-1H-2-benzo-pyran(7),rel-(6R,7R)-5,6,7,8-tetrahydro-7-hydroxy-7-methyl-8-oxo-3-propyl-1H-2-benzopyran(8),scorpinone(9),orsellinic acid(10),6-methyl-1,2,4-benzene-triol(11),2-methyl-5-isopropyl-phenol(12),3-methoxy-5-methyl-phenol(13)and 5-methyl-benzene-1,3-diol(14).Compound 8 and 12 exhibited inhibitory effects on glioblastoma cell(U87)with an IC50 value of 21.07μmol·L-1 and 15.74μmol·L-1 respectively.They also show inhibitory effects on lung cancer cells(A549)with an IC50 value of 20.88μmol·L-1 and 22.93μmol·L-1 respectively.
endophytic fungus of Conus literatusTalaromycessp.secondary metabolitesbioactivities
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