红树老鼠簕内生真菌 Aspergillus terreus GXIMD 03158 次级代谢产物研究
Studies on secondary metabolites of endophytic fungus Aspergillus terreus GXIMD 03158 isolated from mangroves Acanthus ilicifolius L.
安凡 1姜悦 1王宇 1曹广萍 1高程海 1刘永宏 1易湘茜 1白猛1
作者信息
- 1. 广西中医药大学海洋药物研究院/药学院,广西南宁 530200;广西海洋药物重点实验室,广西 南宁 530200
- 折叠
摘要
文章对红树老鼠簕(Acanthus ilicifolius L.)内生真菌Aspergillus terreus GXIMD 03158的抑菌活性次级代谢产物进行研究.采用多种色谱技术对该菌次级代谢产物进行分离纯化,结合核磁氢谱、碳谱和高分辨率质谱数据综合解析鉴定单体化合物结构.从A.terreus GXIMD 03158代谢产物中共分离出10个化合物,经鉴定分别为dankasterones A(1),(14α,22E)-14-hydroxyergosta-4,7,22-triene-3,6-dione(2),steresterone B(3),ergosta-4,6,8(14),22-tetraen-3-one(4),herbarulide(5),demethylincisterol A3(6),isochaetochromin B1(7),isochaetochromin B2(8),(E)-7,9-二烯-11-羰基硬脂酸(9)和aspergilfuranone A(10).并采用微量肉汤稀释法检测所有化合物对白色葡萄球菌(Staphylococcus albus)、金黄色葡萄球菌(Staphylococcus aureus)、表皮葡萄球菌(Staphylococcus epidermidis)、耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus,MRSA)和副溶血弧菌(Vibrio parahaemolyticus)的最低抑菌浓度(minimal inhibitory concentrations,MIC),结果显示化合物6对S.albus、S.aureus和S.epidermidis的MIC分别为3.12、6.25和3.12μg·mL-1;化合物9对S.albus和S.aureus的MIC分别为1.56和3.12μg·mL-1.
Abstract
This work studies the secondary metabolites with antibacterial activity from Aspergillus terreus GXIMD 03158,an endophytic fungus isolated from mangroves Acanthus ilicifolius L.The secondary metabolites of the strains were separated and purified by various chromatographic techniques,and the structures of monomer compounds were identified by comprehensive analyses of 1H,13C NMR and HREIMS date.Ten compounds were isolated from the metabolites of A.terreus GXIMD 03158,and identified as dankasterones A(1),(14α,22E)-14-hydroxyergosta-4,7,22-triene-3,6-dione(2),steresterone B(3),ergosta-4,6,8(14),22-tetraen-3-one(4),herbarulide(5),demethylincisterol A3(6),isochaetochromin B1(7),isochaetochromin B2(8),(E)-7,9-diene-11-carbonyl stearic acid(9)and aspergilfuranone A(10).The minimal inhibitory concentrations(MIC)of all compounds against Staphylococcus albus,Staphylococcus aureus,Staphylococcus epidermidis,Methicillin-resistant Staphylococcus aureus and Vibrio parahaemolyticus were determined by broth microdilution method,the results showed that compound 6 exhibited significant antibacterial activity against S.albus,S.aureus and S.epidermidis,and the MIC values were 3.12,6.25 and 3.12 μg·mL-1,respectively;compound 9 exhibited significant antibacterial activity against S.albus and S.aureus,and the MIC values were 1.56 and 3.12 μg·mL-1,respectively.This study provides a theoretical basis for the research on development and utilization of mangrove Acanthus ilicifolius L.and its endophytic fungi.
关键词
红树老鼠簕/内生真菌/曲霉属真菌/次级代谢产物/抑菌活性Key words
mangroves Acanthus ilicifolius L./endophytic fungi/Aspergillus terreus/secondary metabolites/antibacterial activities引用本文复制引用
出版年
2024
NETL
NSTL
万方数据