Studies on secondary metabolites of endophytic fungus Aspergillus terreus GXIMD 03158 isolated from mangroves Acanthus ilicifolius L.
This work studies the secondary metabolites with antibacterial activity from Aspergillus terreus GXIMD 03158,an endophytic fungus isolated from mangroves Acanthus ilicifolius L.The secondary metabolites of the strains were separated and purified by various chromatographic techniques,and the structures of monomer compounds were identified by comprehensive analyses of 1H,13C NMR and HREIMS date.Ten compounds were isolated from the metabolites of A.terreus GXIMD 03158,and identified as dankasterones A(1),(14α,22E)-14-hydroxyergosta-4,7,22-triene-3,6-dione(2),steresterone B(3),ergosta-4,6,8(14),22-tetraen-3-one(4),herbarulide(5),demethylincisterol A3(6),isochaetochromin B1(7),isochaetochromin B2(8),(E)-7,9-diene-11-carbonyl stearic acid(9)and aspergilfuranone A(10).The minimal inhibitory concentrations(MIC)of all compounds against Staphylococcus albus,Staphylococcus aureus,Staphylococcus epidermidis,Methicillin-resistant Staphylococcus aureus and Vibrio parahaemolyticus were determined by broth microdilution method,the results showed that compound 6 exhibited significant antibacterial activity against S.albus,S.aureus and S.epidermidis,and the MIC values were 3.12,6.25 and 3.12 μg·mL-1,respectively;compound 9 exhibited significant antibacterial activity against S.albus and S.aureus,and the MIC values were 1.56 and 3.12 μg·mL-1,respectively.This study provides a theoretical basis for the research on development and utilization of mangrove Acanthus ilicifolius L.and its endophytic fungi.
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