低温诱导大百合鳞茎休眠解除与酚类代谢关系
Association Between Dormancy Release and Phenolic Metabolic Alteration Induced by Low Temperature in Cardiocrinum giganteum Bulbs
蔡子安 1李荣琛 1赵志珩 2陈玉珍 1卢存福1
作者信息
- 1. 北京林业大学生物科学与技术学院,林木育种与生态修复国家工程研究中心,北京 100083
- 2. 广西壮族自治区林业科学研究院,广西特色经济林重点实验室,南宁 530002
- 折叠
摘要
大百合(Cardiocrinum giganteum)为多年生球根药食同源植物,其鳞茎具有典型的生理休眠特性,而低温是百合鳞茎解除休眠的重要环境因子.为揭示大百合鳞茎休眠解除的分子机制,该研究对 4℃低温处理 0、30 和 60 d的鳞茎分别进行代谢组和转录组分析.结果表明,鳞茎休眠的解除与酚类物质的代谢相关,酚类物质的降解有利于解除休眠,其中苯丙氨酸解氨酶基因(PALs)在此过程中可能起主要作用.同时,bHLH、bZIP、MYB 和MADS等转录因子家族成员均与酚类代谢物显著相关,且参与解除休眠.共表达分析证实PAL、CAD 和POD是酚类代谢重要的调控基因,MYB4、MYB114 和ICE1 参与了酚类代谢调控网络,其中ICE1可能是连接温度信号和酚类代谢的关键因素.这些转录因子与酚类物质的共同作用可能对鳞茎打破休眠具有重要作用.
Abstract
Cardiocrinum giganteum,a perennial bulbous plant of family Liliaceaeas,is an important medicinal and food homologous plant.The bulbs have typical dormancy characteristics,and low temperature is a major environmental factor to relieve dormancy of lily bulbs.In order to reveal the molecular mechanism of dormancy dissolution of Lily bulbs,metabolome and transcriptome analysis were performed on bulbs treated at 4℃ for 0,30 and 60 days,respectively.The results showed that dormancy release of bud was related to the metabolism of phenols,and the degradation of phenols was beneficial to the release of dormancy,among which PALs gene might play a major role in this process.Meanwhile,members of transcription factor families such as bHLH,bZIP,MYB and MADS were all significantly related with phenolic metabolites and involved in bulb dormancy release.Co-expression analysis further confirmed that PAL,CAD and POD were important regulatory genes for phenolic metabolism,MYB4,MYB114 and ICE1 were involved in the regulatory network of phenolic metabolism,and ICE1 might be the key factor connecting temperature signals and phenolic metabolism.Therefore,the interaction of these transcription factors with phenols might play an important role in breaking dormancy of bulbs.
关键词
低温/酚类/休眠解除/转录组/代谢组/共表达Key words
Low temperature/Phenols/Dormancy release/Metabolome/Transcriptome/Co-expression引用本文复制引用
基金项目
广西特色经济林培育与利用重点实验室开放基金(19-B-01-02)
广西壮族自治区创新驱动发展专项子课题(桂科AA17204058-03)
北京林业大学优秀研究生导师团队建设项目(YJSY-DSTD2022005)
出版年
2024
国家科技期刊平台
NSTL
万方数据