摘要
为了解重唇石斛(Dendrobium hercoglossum)的化学成分,利用硅胶、Sephadex LH-20柱色谱和半制备高效液相色谱等方法,从其地上部分分离了14 个化合物,经波谱分析,其结构分别鉴定为:3,4,5-三甲氧基苯酚(1)、3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-propan-1-one(2)、methyl 3-(p-hydroxyphenyl)propionate(3)、justiciresinol(4)、榕醛(5)、异落叶松脂素(6)、threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-methoxypropanol(7)、(+)-松脂素(8)、aloifol I(9)、dendrocandin T(10)、3-甲氧基-4-羟基苯丙酮(11)、1,3,5-三甲氧基苯酚(12)、香草醇(13)和丁香脂素(14),其中化合物 1～12,14 均为首次从重唇石斛中分离得到,化合物7、10 和14 为首次从石斛属植物中分离得到.化合物4、8 和10 具有显著的酪氨酸酶抑制活性,IC50值分别为(10.40±0.36)、(12.16±0.18)和(9.46±0.78)μmol/L.
Abstract
In order to understand the chemical constituents of Dendrobium hercoglossum,fourteen compounds were isolated from the aerial parts of D.hercoglossum by using various column chromatography with silica gel,sephadex LH-20,and semi-preparative HPLC.Based on spectroscopic data,their structures were identified as 3,4,5-trimethoxyphenol(1),3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-propan-1-one(2),methyl 3-(p-hydro-xyphenyl)propionate(3),justiciresinol(4),ficusal(5),meso-secoisolariciresinol(6),threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-methoxypropanol(7),(+)-pinoresinol(8),aloifol I(9),dendrocandins T(10),3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-propan-1-one(11),1,3,5-trimethoxybenzene(12),homovanillyl alcohol(13)and(-)-syringaresinol(14).Compounds 1-12,14 were obtained from D.hercoglossum for the first time,and compounds 7,10,and 14 were isolated from Dendrobium species for the first time.The compounds 4,8 and 10 showed significant inhibitory activity,with IC50 of(10.40±0.36),(12.16±0.18)and(9.46±0.78)μmol/L,respectively.
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