Genome-wide Identification and Expression of FER Gene Family in Manihot esculenta
Plant ferritin(FER)can store iron and respond to various abiotic stresses.The FER gene family of cassava(Manihot esculenta)was analyzed based on a genome-wide level.The results showed that four FER genes had been identified from cassava,named MeFERs,encoded MeFERs could be divided into two clades based on phylogenetic tree analysis.All members contained Euk_Ferritin functional domain,and located in the chloroplast.The MeFERs genes located in chromosome LG7-LG10,respectively.Gene collinearity analysis showed that there were three potential duplicate gene pairs without tandem duplication events.The Ka/Ks value indicated that the homologous gene of MeFER was purified and selected.The family contained cis-acting elements in response to hormones and stress induction.Moreover,qRT-PCR analysis showed that the expression of MeFERs gene was tissue-specific,and MeFER4 gene responded to various stresses,with the most significant response under drought stress.Therefore,these would provide a foundation for future functional studies of the cassava FER gene family.
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