摘要
为了解金钗石斛乙醇提取物正丁醇部位的化学成分,通过硅胶、凝胶、反相硅胶和高效液相等各种色谱技术进行分离纯化得到 3 个化合物,并根据核磁共振、质谱等波谱手段确定其结构分别为 2,11-epoxy-11,13-dihydroxypicrotoxano-3(15)-lactone(1)、2,4,6-三甲氧基苯-1-O-β-D-葡萄糖苷(2)和3-methylbutan-1-ol β-D-glucopyranoside(3).其中化合物1 是一个新的木防己毒烷型倍半萜,该类化合物是金钗石斛中具有代表性的倍半萜类型.所有化合物均没有 α-葡萄糖苷酶和酪氨酸酶抑制活性,同时化合物1 也没有神经保护活性.
Abstract
In order to study the chemical constituents in butanol fraction of ethanol extract of Dendrobium nobile,column chromatographic techniques including silica gel,Sephadex LH-20,revised silica gel and HPLC were used to separate the compounds,and spectroscopic techniques including NMR and MS were implied to determine the structures.Totally 3 compounds were isolated,and their structures were identified as 2,11-epoxy-11,13-dihydro-xypicrotoxano-3(15)-lactone(1),2,4,6-trimethoxyphenol-1-O-β-D-glycoside(2),3-methylbutan-1-ol β-D-gluco-pyranoside(3).Compound 1 was a new picrotoxane-type sesquiterpene,which is one of the representative components in D.nobile.However,none of the compounds showed α-glucosidase and tyrosinase inhibitory activity,none of them showed activity,and compound 1 show no neuroprotective activity either.
基金项目
海南省院士创新平台科研专项(YSPTZX202037)
现代农业产业技术体系建设项目(CARS-21)
国家科技期刊平台
NSTL
万方数据