Genetic Diversity of Hevea brasiliensis by EST-SSR Markers
Nineteen pairs of EST-SSRs primers were employed to detect the genetic diversity of 41 accessions of Hevea brasiliensis germplasm(6 wild accessions and 35 cultivars). These 19 pairs of EST-SSRs primers resulted in expected amplified products by amplifying 92 polymorphic loci, and each primer detected 2 to 7 alleles or 4.84 alleles by average. At the similarity coefficient level of 0.64, wild accessions and cultivars were segregated; at the similarity coefficient level of 0.75, the eultivars were divided into four groups; the genetic distance between cuhivars and wild accessions ranged from 0.11 to 1.16, with a shorter genetic distance among the cuhivars and a longer genetic distance among the wild accessions. The longest genetic distance between the cuhivars and wild accessions was 1.16, which was between AC/T/15/114 and PR261, and the shortest one was 0.11, which was between Baoting 155 and Reyan 7-20-59.