The genetic diversity and relationship of sisal were analyzed by fluorescent AFLP technology. AFLP fingerprinting of 40 accessions with eight pairs of Pst I/Mse I primers revealed a total number of 785 unambiguous bands including 507 polymorphic bands, and the polymorphism frequency was 64.58％. Each pair of primers produced 98.13 polymorphic bands on average. The primer P-GAC/M-CAC had the highest polymorphism frequency (78.63％), followed by the primer P-GTG/M-CTC (71.28％). The primer P-GTA/M-CTT had the least polymorphism frequency(52.94％). These show there are abundant diversities about enzyme digestion sites in theaccessions. The similarity coefficient of the 40 accessions ranged from 0.44 to 0.83. The 40 accessions were divided into 4 groups at the similarity coefficient of 0.51. Moreover, some specific bands were detected from the 40 accessions by using 8 pairs of primers, and could be used to distinguish the accessions under test.
国家科技期刊平台
NSTL
万方数据
维普
