Genetic Diversity Analysis of Sclerotium rolfsii Sacc.in Rhizoma of Atractylodes Macrocephala
The genetic diversity of Sclerotium rolfsii Sacc. in Atractylodes macrocephala was studied in order to analyse the relationship between the strains from 5 regions (Guiyang, Zunyi, Qiandongnan, Qiannan and Bijie city)in Guizhou Province. 20 strains collected from different biotopes were amplified by 116 RAPD primers. The genetic relationships were generated using UPGMA method of NTSYS software based on the DNA fingerprint. 64 bands including 44 reproducible polymorphic bands with 68.75% polymorphic band frequency based on 12 RAPD primers were obtained . The similarity coefficient between strains was from 0.56 to 0.98, which were divided into 3 groups marked I, II and M based the threshold of similarity coefficient at 0.79. The results indicated that DNA polymorphism had no significant correlation with geographical origins, excepting a few of strains from Bijie were divided into a same group.
国家科技期刊平台
NSTL
万方数据
维普
