Effects of Extraneous Spermidine on the Salt Tolerance in Cucumber Seedlings under Salt Stress
In order to explore the effect of different concentrations of extraneous spermidine on the salt tolerance in cucumber seedlings under salt stress,"JinChun No.4" was used in the artificial climate incubator with matrix culture methods,and different concentrations of extraneous spermidine (0,50,100,150,200,250 mg/L)were applied at cucumber seedlings under 200 mmol/L NaCl stress to study the changes of the cucumber seedlings growth,chlorophyll content,malondialdehyde(MDA)content,relative conductivity,soluble sugar content,and sucrose content.The results indicated that a certain concentration of extraneous spermidine increased significantly the seedling morphological indicators and chlorophyll content; the treatment of 100 mg/L extraneous spermidine decreased significantly the content of MDA in seedling leaves,the largest drop can reach to 47.4%; the treatment of 100 mg/L extraneous spermidine decreased significantly the relative conductivity; a higher concentration of extraneous spermidine could significantly improve the content of soluble sugar,a lower concentration of extraneous spermidine could significantly improve the content of sucrose content.Based on the physiological indices,it could be concluded that a certain concentration of extraneous spermidine could significantly relieve the salt injury and improve salt tolerance of the cucumber seedlings.