利用15对SSR引物,分析具有广泛来源的70份可可资源的遗传多样性.结果表明,15对引物共扩增出76个条带,其中50个多态性条带,占总带数的65.8％.70份可可资源间遗传相似系数(SM)在0.341 ～0.943之间,平均值为0.625,说明可可资源具有丰富的遗传多样性.在相似系数为0.65水平上,可将70份可可资源分成10类.利用Structure 2.3.2软件分析群体结构,结合可可果实相关性状的表型数据,采用Tassel 2.1的一般线性模型(General linear model,GLM)进行关联分析;结果表明18个位点与果重、果壳重、果长、果径围、果壳厚显著相关(p＜0.05),各位点对表型变异贡献率为5.5％～13.1％.
Genetic Diversity and Association Analysis of Cacao Germplasm Using SSR Markers
Fifteen SSR primers were used to study the genetic diversity among wide original 70 cacao germplasm resources.Seventy-six loci were detected by the 15 primers,of which 50 were polymorphic and its polymorphic ratio was amounted for 65.8％.The genetic similarity was ranged from 0.341 to 0.943 among the accessions,with an average of 0.625.The results indicated that the cacao accessions were rich in genetic diversity.Based on the dendrogram of UPGMA,70 accessions could be divided into ten groups at the level of 0.65.Based on the analysis of population structure,an association analysis between SSR markers and pod agronomic traits were performed using the Tassel 2.1 GLM (general linear model) program.Of the SSR loci,18 were significantly associated with pod weight,pod shell weight,pod length,pod equatorial circumference,and pod shell thickness under GLM program,and the ratio of explanation on the phenotype variation of related markers ranged from 5.5％-13.1％.
国家科技期刊平台
NSTL
万方数据
维普
