Induction of Friable Callus and the Establishment of Suspension Cell Lines of Camellia nitidissima Chi
The stem,leaf,anther and somatic embryo of Camellia nitidissima Chi were used as the materials in this study.With the methods of tissue culture and microscopic observation,the induction of friable callus and establishment of suspension cell lines were conducted.The study showed the optimal process to obtain friable callus from C.nitidissima chi.According to this work,the slices of somatic embryo were cultured on the MS medium supplemented with 0.5 mg/L KT,8.0 mg/L NAA,5 mg/L AgNO3 and 30 g/L sucrose under dark condition to induce callus.The newly induced calli were separated from somatic embryo and were cultured on the callus inducing medium for three months.Finally,the friable calli were subcultured on the media containing inositol or AgNO3 alternatively;According to analysis of variance,the content of sucrose,inositol and AgNO3 significantly affected the cell density in suspension cultured liquid medium of C.nitidissima Chi and the lighting condition had significant interaction with the last two;The optimal process to establish suspension cell lines of C.nitidissima was also reported in this paper.In this process the friable callus were cultured alternatively in liquid proliferation medium containing 100 mg/L inositol or 2.5 mg/L AgNO3 under dark condition.This work can be basis for large-scale cell culture of C.nitidissima Chi.
国家科技期刊平台
NSTL
万方数据
维普
