Phylogenetic Analysis of Beta-amylase Gene Family in Musa accuminata
Beta-amylase (Bmy) is a key starch-degrading enzyme,plays an important role in the process of banana fruit ripening starch degradation into soluble sugar.In order to get a full overview of MaBmy gene in the banana genome,the basic physical and chemical characters,secondary structure,subcellular location,gene structure,evolutionary relationship and conserved motif of MaBmy genes family were conducted by the bioinformatics methods.The results showed that there were 15 genes annotated to beta-amylase.The 15 putative MaBmy were named MaBmy1~15 based on the chromosomal location.The identified MaBmy gene family members encoded predicted proteins ranged from 68 to 1 453 amino acid residues (aa) in length,and computed molecular masses of 7.8 to 162.6 ku,there were 10 acidic proteins and 5 alkalinity proteins in the MaBmy family.Instability index analysis found that 6 of the MaBmy protein were stabilized protein.Hydrophobic analysis showed that all of the MaBmy proteins were hydrophilic protein.MaBmy genes family contained at least two introns,with the most 10 introns.Evolution analysis indicated that MaBmy family cuold be grouped into four subfamilies,and the phylogenetic relationship between banana and rice was relatively closer.This study provied a blue-print for their functional charaterization and utilization in banana furit ripening improvement.